Biomasse

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Biomasse

Biomasse : paroi, cellulose et polyphénols

La plateforme a développé et mis en place différents outils d'analyse de la paroi appliqués à l'étude à moyen débit de mutants et de lignées recombinantes.

Nous avons ainsi mis en place des techniques émergentes d’empreinte enzymatique dans le domaine de l’analyse des polysaccharides pariétaux, impliquant l’utilisation du MALDI/TOF.

Ces techniques sont complémentaires de celles également utilisées au laboratoire comme la FTIR, la chromatographie ionique HPAEC ou l’analyse par GC/MS après dérivatisation, et deviennent rapidement incontournables pour l’étude des polysaccharides.

Par ailleurs, le suivi des synthèses des phénols et polymères phénoliques étudiés par l'équipe Biopolymères lignocellulosiques passe nécessairement par les techniques LC-MS/MS et MALDI-TOF, outils indispensables à leur identification par analyse structurale et détermination des masses exactes.

Voir aussi

Publications incluant des analyses de parois effectuées sur le plateau de chimie :

  • Zhao, F., Chen, W., Sechet, J., Martin, M., Bovio, S., Lionnet, C., Long, Y., Battu, V., Mouille, G., Monéger, F., Traas, J. (2019) Xyloglucans and Microtubules Synergistically Maintain Meristem Geometry and Phyllotaxis. Plant Physiology Nov 2019, 181 (3) 1191-1206; DOI: 10.1104/pp.19.00608
  • Lapierre C, Voxeur A, Boutet S, Ralph J (2019) Arabinose Conjugates Diagnostic of Ferulate-Ferulate and Ferulate-Monolignol Cross-Coupling Are Released by Mild Acidolysis of Grass Cell Walls .J Agric Food Chem. 67(46):12962-12971. doi: 10.1021/acs.jafc.9b05840
  • Strobl, S. M., Kischka, D., Heilmann, I., Mouille, G. and Schneider, S. (2018) The Tonoplastic Inositol Transporter INT1 From Arabidopsis thaliana Impacts Cell Elongation in a Sucrose-Dependent Way. Front. Plant Sci. 9 1657. DOI 10.3389/fpls.2018.01657
  • Gonçalves, B., Maugarny-Calès, A., Adroher, B., Cortizo, M., Borrega, N., Blein, T., Hasson, A., Gineau, E., Mouille, G., Laufs, P. and Arnaud, N. (2017) GDP-L-fucose is required for boundary definition in plants. J Exp Bot, erx402, https://doi.org/10.1093/jxb/erx402
  • Xu, Y., Sechet, J., Wu, Y., Fu, Y., Zhu, L., Li, J., Zhang, Y., Gineau, E., Gaertner, C., Zhou, J., Fan, X., Liu, Y., Zhou, L., Mouille, G., Lin, X.(2017) Rice sucrose partitioning mediated by a putative pectin methyltransferase and homogalacturonan methylesterification. Plant Physiol. 2017 May 11. pii: pp.01555.2016. doi: 10.1104/pp.16.01555
  • Chupin, L., de Ridder, D., Clément-Vidal, A., Soutiras, A., Gineau, E., Mouille, G., Arnoult, S., Brancourt-Hulmel, M., Lapierre, C. (2017) Influence of the radial stem composition on the thermal behaviour of miscanthus and sorghum genotypes. Carbohydrate Polymer dx.doi.org/10.1016/j.carbpol.2017.03.002
  • Roongsattham, P., Morcillo, F., Fooyontphanich, K., Jantasuriyarat, C., Tragoonrung, S., Amblard, P., Collin, M., Mouille, G., Verdeil, J.-L. and Tranbarger, T. J. (2016) Cellular and Pectin Dynamics during Abscission Zone Development and Ripe Fruit Abscission of the Monocot Oil Palm. Front. Plant Sci., 26 April 2016 | http://dx.doi.org/10.3389/fpls.2016.00540
  • Verger, S., Chabout, S., Gineau, E., Mouille G. (2016) Cell adhesion in plants is under the control of putative O-fucosyltransferases. Development doi: 10.1242/dev.132308
  • Sechet, J., Frey, A., Cuzzi, D., Berger, A., Perreau, F., Cueff, G., Charif, D., Rajjou, L., Mouille, G., North, H.M. and Marion-Poll, A.(2016) Xyloglucan metabolism differentially impacts the cell wall characteristics of the endosperm and embryo during Arabidopsis seed germination. Plant Physiol. First Published on January 29, 2016, doi: http:/​/​dx.​doi.​org/​10.​1104/​pp.​15.​01312
  • Pawar, P. M.-A., Derba-Maceluch, M., Chong, S.-L., Gómez, L. D., Miedes, E., Banasiak, A., Ratke, C., Gaertner, C., Mouille, G., McQueen-Mason, S. J., Molina, A., Sellstedt, A., Tenkanen, M. and Mellerowicz, E. J.(2016) Expression of fungal acetyl xylan esterase in Arabidopsis thaliana improves saccharification of stem lignocellulose. Plant Biotechnol J 14 (1) 1467-7652 http://dx.doi.org/10.1111/pbi.12393
  • Adler, S., Mouille, G., Fock-Bastide, I., Citerne, S., Noirot M. (2016) Comparison between Coffea arabica L. ‘Laurina’ and C. arabica ‘Bourbon’ seedlings grown in daylight or darkness for their polysaccharidic cell wall composition and caffeine and chlorogenic acid contents. Trees. First online: 23 December 2015. (doi:10.​1007/​s00468-015-1309-x)
  • Adler, S., Noirot, M., Fock-Bastide, I., Citerne, S., Mouille, G. (2015) Variations in cell wall monosaccharide composition during seed development in Coffea arabica L. Comparison between Coffea arabica var. Bourbon and Coffea arabica var. Laurina. Trees http://dx.doi.org/10.1007/s00468-015-1268-2
  • Bensussan, M., Lefebvre, V., Ducamp, A., Trouverie , J., Gineau, E., Fortabat, M.-N., Guillebaux, A., Baldy, A., Naquin, D., Herbette, S., Lapierre, C., Mouille, G., Horlow, C., Durand-Tardif, M. (2015) Suppression of dwarf and irregular xylem phenotypes generates low acetylated biomass lines in Arabidopsis thaliana. Plant Physiol,  Published online before print April 2015, doi: http:/​/​dx.​doi.​org/​10.​1104/​pp.​15.​00122
  • Draeger, C., Fabrice, T. N., Gineau, E., Mouille, G., Kuhn, B. M., Moller, I., Abdou, M.-T., Frey, B., Pauly, M., Bacic, A. and Ringli, C. (2015) Arabidopsis leucine-rich repeat extensin (LRX) proteins modify cell wall composition and influence plant growth. BMC Plant Biology 15:155 DOI: 10.1186/s12870-015-0548-8
  • Boron, A. K., Van Orden, J., Markakis, M. N., Mouille, G., Adriaensen, D., Verbelen, J.-P., Höfte, H. and Vissenberg, K. (2014) Proline-rich protein-like PRPL1 controls elongation of root hairs in Arabidopsis thaliana J. Exp. Bot. doi: 10.1093/jxb/eru308 First published online: August 21, 2014
  • Sénéchal, F., Graff, L., Surcouf, O., Marcelo, P., Rayon, C., Bouton, S., Mareck, A., Mouille, G., Stintzi, A., Höfte, H., Lerouge, P., Schaller, A. and Pelloux, J.(2014) Arabidopsis PECTIN METHYLESTERASE17 is co-expressed with and processed by SBT3.5, a subtilisin-like serine protease Ann Bot doi: 10.1093/aob/mcu035 First published online: March 24, 2014
  • Timpano, H., Sibout, R., Devaux, M.-F., Alvarado, C., Looten, R., Falourd, Pontoire, B., Martin, M., Legée, F., Cézard, L., Lapierre, C., Badel, E., Citerne, S., Vernhettes, S., Höfte, H., Guillon, F., Gonneau M. (2014) Brachypodium Cell Wall Mutant with Enhanced Saccharification Potential Despite Increased Lignin Content, Bioenerg Res, DOI 10.1007/s12155-014-9501-1 .
  • Paque, S., Mouille, G., Grandont, L., Alabadi, D., Gaertner, C., Goyallon, A., Muller, P., Primard-Brisset, A., Sormani, R., Blazquez, M.A., Perrot-Rechenmann, C. (2014). AUXIN BINDING PROTEIN1 Links Cell Wall Remodeling, Auxin Signaling, and Cell Expansion in Arabidopsis. Plant Cell 26(1), 280-95.
  • Gendre, D., McFarlane, H. E., Johnson, E., Mouille, G., Sjödin, A., Oh, J., Levesque-Tremblay, G., Watanabe, Y., Samuels, L., Bhalerao,R. P. (2013) Trans-Golgi Network Localized ECHIDNA/Ypt Interacting Protein Complex Is Required for the Secretion of Cell Wall Polysaccharides in Arabidopsis. Plant Cell 25(7), 2633-2646.
  • Geshi, N., Johansen, J. N., Dilokpimol, A., Rolland, A., Belcram, K., Verger, S., Kotake, T., Tsumuraya, Y., Kaneko, S., Tryfona, T., Dupree, P., Scheller, H. V., Höfte, H. and Mouille, G. (2013), A galactosyltransferase acting on arabinogalactan protein glycans is essential for embryo development in Arabidopsis. Plant J 76 (1), 128-137.
  • Sipponen, M. H., Lapierre, C., Méchin, V., & Baumberger, S. (2013). Isolation of structurally distinct lignin-carbohydrate fractions from maize stem by sequential alkaline extractions and endoglucanase treatment. Bioresour Technol 133 522-528.

Date de modification : 03 juillet 2023 | Date de création : 28 septembre 2009 | Rédaction : AMG-FP