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Unité Bioger (UMR 1290) Team Effecteurs et pathogenèse chez Leptosphaeria maculans Bâtiment 13, av. Lucien Brétignières, BP01 78850 Thiverval-Grignon   Tél. : +33 (0)1 30 81 45 90 Fax : +33 (0)1 30 81 53 06 Mail : Isabelle.Fudal@inrae.fr

Research

I am a research scientist hired at INRA in the BIOGER unit in 2006, with a background in plant pathology, molecular biology and functional genomics. My main focus was, and still is, on deciphering fungal-plant interactions. Most of my research experience was devoted to understand fungal pathogenicity and signals produced by the fungus, which induce recognition of “non-self” and disease resistance (avirulence genes or effectors). Since I was hired as a permanent scientist at INRA, I am developing functional genomics approaches on plant-fungi interactions with a particular interest to fungal effectors on the model of interaction Leptosphaeria maculans / Brassica napus. The objective of my research project is to study the possible roles of effectors on pathogenicity towards B. napus (identification, expression, regulation, evolution and functional analysis). To achieve this goal, I develop a pluri-disciplinary project combining bioinformatics, transcriptomics, functional genomics, pathogenicity studies and evolutionary genomics. Recently, I focused part of my researches on the determinism of concerted regulation of effector genes during infection and pointed out on the importance of chromatin status in this regulation. I am co-leading the INRA SPE network REacTION (network on the epigenetic mechanisms that shape plant - Bioaggressors and Symbiotic Organisms interactions)

Curriculum vitae

Publications

Clairet C, Lapalu N, Simon A, Soyer JL, Viaud M, Zehraoui E, Dalmais B, Fudal I, Ponts N (2021) Nucleosome patterns in four plant pathogenic fungi with contrasted genome structures. BioRxiv doi: https://doi.org/10.1101/2021.04.16.439968

Gay EJ, Soyer JL, Lapalu N, Linglin J, Fudal I, Da Silva C, Wincker P, Aury JM, Cruaud C, Levrel A, Lemoine J, Delourme R, Rouxel T, Balesdent MH (2021) Large-scale transcriptomics to dissect two years of the life of a fungal phytopathogen interacting with its host plant. BMC Biol 19: 55

Jiquel A, Gervais J, Geistodt-Kiener A, Delourme R, Gay EJ, Ollivier B, Fudal I, Faure S, Balesdent MH, Rouxel T (2021) A gene-for-gene interaction involving a ‘late’ effector
contributes to quantitative resistance to the stem canker disease in Brassica napus. New Phytol https://doi: 10.1111/nph.17292

Soyer JL, Clairet C, Gay EJ, Lapalu N, Rouxel T, Stukenbrock EH, Fudal I (2021). Genome-wide mapping of histone modifications in two species of Leptosphaeria maculans showing contrasting genomic organization and host specialization. Chromosome Res https://doi.org/10.1007/s10577-021-09658-1

Lazar N, Mesarich CH, Petit-Houdenot Y, Talbi N, Li de la Sierra-Gallay I, Zélie E, Blondeau K, Gracy J, Ollivier B, Blaise F, Rouxel T, Balesdent MH, Idnurm A, van Tilbeurgh H, Fudal I (2020) A new family of structurally conserved fungal effectors displays epistatic interactions with plant resistance proteins. bioRxiv https://doi.org/10.1101/2020.12.17.423041

Rocafort M., Fudal I. and Mesarich C. H. (2020). Apoplastic effector proteins of plant-associated fungi and oomycetes. Current Opinion in Plant Biology  56: 9-19. https://doi.org/10.1016/j.pbi.2020.02.004.

Petit-Houdenot Y., Degrave A., Meyer M., Blaise F., Ollivier B., Marais C.L., Jauneau A., Audran C., Rivas S., Veneault-Fourrey C., Brun H., Rouxel T., Fudal I. and Balesdent M.H (2019). A two genes for one gene interaction between Leptosphaeria maculans and Brassica napus. New Phytologist 223: 397-411. DOI: 10.1111/nph.15762

Fudal I., Balesdent M.H., Rouxel T. (2018). Effector biology in fungal pathogens of nonmodel crop plants. Trends in Plant Science.  DOI: https://doi.org/10.1016/j.tplants.2018.07.002

Sanchez-Vallet A., Fouché S., Fudal I., Hartmann F.E., Soyer J.L., Tellier A., Croll D. (2018). The genome biology of effector gene evolution in filamentous plant pathogens. Annual Review of Phytopathology  56: DOI: 10.1146/annurev-phyto-080516-035303

Petit-Houdenot Y., Fudal I. (2017). Complex interactions between fungal avirulence genes and their corresponding plant resistance genes and consequences for disease resistance management. Frontiers in Plant Science  8:1072 ; DOI: 10.3389/fpls.2017.01072

Gervais J., Plissonneau C., Linglin J., Meyer M., Labadie K., Cruaud C., Fudal I., Rouxel T., Balesdent M.H. (2017). Different waves of effector genes with contrasted genomic location are expressed by Leptosphaeria maculans during cotyledon and stem colonisation of oilseed rape. Molecular Plant Pathology  18: 1113-1126. DOI: 10.1111/mpp.12464

Plissonneau C., Daverdin G., Ollivier B., Blaise F., Degrave A., Fudal I., Rouxel T. and Baldesdent M.H. (2016). A game of hide and seek between avirulence genes AvrLm4-7 and AvrLm3 in Leptosphaeria maculans. New Phytologist  209: 1613-1624. DOI: 10.1111/nph.13736

de Marchi R., Sorel M., Mooney B., Fudal I., Goslin K., Kwasniewsk, K., Ryan P. T., Pfalz M., Kroymann J., Pollmann S., Feechan A., Wellmer F., Rivas S., Graciet E. (2016). The N-end rule pathway regulates pathogen responses in plants. Scientific Reports  6. DOI : 10.1038/srep26020

SoyerJ.L., Rouxel T. and Fudal I. (2015). Chromatin-based control of effector gene expression in plant-associated fungi. Current Opinion in Plant Biology  26:51-56. DOI:10.1016/j.pbi.2015.05.025

Blondeau K., Blaise F., Graille M., Kale S.D., Linglin J., Ollivier B., Labarde A., Lazar N., Daverdin G., Balesdent M.H., Choi D.H.Y., Tyler B., Rouxel T., van Tilbeurgh H. and Fudal I. (2015). The avirulence gene AvrLm4-7 of Leptosphaeria maculans: linking crystal structure to functional and adaptive characteristics. The Plant Journal  83(4):610-624.

Soyer J.L., Hamiot A., Ollivier B., Balesdent M.H., Rouxel T. and Fudal I. (2015). The APSES transcription factor LmStuA is required for sporulation, pathogenic development and effector gene expression in Leptosphaeria maculans. Molecular Plant Pathology : doi: 10.1111/mpp.12249.

Ghanbarnia K., Fudal I., Larkan N.J., Links M.G., Balesdent M.H., Profotova B., Fernando W.G.D., Rouxel T. and Borhan M.H. (2015). Rapid identification of the Leptosphaeria maculans avirulence gene AvrLm2 using an intraspecific comparative genomics approach. Molecular Plant Pathology  16(7):699-709. DOI: 10.1111/mpp.12228

Grandaubert J., Lowe R.G.T., Soyer J.L., Schoch C.L., Van de Wouw A.P., Fudal I., Robbertse B., Lapalu N., Links M.G., Ollivier B., Linglin J., Barbe V., Mangenot S., Cruaud C., Borhan H., Howlett B.J., Balesdent M.H. and Rouxel T. (2014). Transposable Element-assisted evolution and adaptation within the Leptosphaeria maculans-Leptosphaeria biglobosa species complex of fungal plant pathogens. BMC Genomics  15(1):891; https://doi.org/10.1186/1471-2164-15-891

Soyer J.L., El Ghalid M., Glaser N., Ollivier B., Linglin J., Grandaubert J., Balesdent M.H., Connolly L.R., Freitag M., Rouxel T. and Fudal I (2014). Epigenetic control of effector gene expression in the plant pathogenic fungus Leptosphaeria maculans. PLOS Genetics  10(3): e1004227. https://doi.org/10.1371/journal.pgen.1004227

Delourme R., Bousset L., Ermel E., Duffé P., Besnard A.L., Marquer B., Fudal I., Linglin J., Chadoeuf J. and Brun H (2014). Quantitative resistance affects the speed of frequency increase but not the diversity of the virulence alleles overcoming a major resistance gene to Leptosphaeria maculans in oilseed rape. Infection, Genetics and Evolution  https://doi.org/10.1016/j.meegid.2013.12.019

Tyler B.M., Kale S.D., Wang Q., Tao K., Clark H.R., Drew K., Antignani V., Rumore A., Hayes T., Plett J.M., Fudal I., Gu B., Chen Q., Affeldt K.J., Berthier E., Fischer G.J., Dou D., Shan W., Keller N., Martin F., Rouxel R., Lawrence C.B. (2013). Microbe-independent entry of oomycete RxLR effectors and fungal RxLR-like effectors into plant and animal cells is specific and reproducible. Molecular Plant-Microbe Interactions  26(6): 611-616. https://doi.org/10.1094/MPMI-02-13-0051-IA

Balesdent M.H., Fudal I., Ollivier B., Bally P., Grandaubert J., Eber F., Chevre A.M., Leflon M. and Rouxel T. (2013) The dispensable chromosome of Leptosphaeria maculans shelters an effector gene conferring avirulence towards Brassica rapa. New Phytologist  198: 887-898. https://doi.org/10.1111/nph.12178

Daverdin G., Rouxel T., Gout L., Aubertot J.N., Fudal I., Meyer M., Parlange F., Carpezat J. and Balesdent M.H. (2012). Genome Structure and Reproductive Behaviour Influence the Evolutionary Potential of a Fungal Phytopathogen. PLOS Pathogens  8(11): e1003020. https://doi.org/10.1371/journal.ppat.1003020

Bourras S., Meyer M., Grandaubert J., Lapalu N., Fudal I., Linglin J., Ollivier B., Blaise F., Balesdent M.H. and Rouxel T. (2012). Incidence of Genome Structure, DNA Asymmetry and Cell Physiology on T-DNA Integration in Chromosomes of the Phytopathogenic Fungus Leptosphaeria maculans. Genes Genomes Genetics  2: 891-904. https://doi.org/10.1534/g3.112.002048

Rouxel T., Grandaubert J., Hane J.K., Hoede C., van de Wouw  A.P., Couloux  A., Dominguez V., Anthouard V., Bally P., Bourras S., Cozijnsen A.J., Ciuffetti L.M., Degrave A., Dilmaghani A., Duret L., Fudal I., Goodwin S.B., Gout L., Glaser N., Linglin J., Kema G.H.G., Lapalu N., Lawrence C.B., May K., Meyer M., Ollivier B., Poulain J., Simon A., Stachowiak A., Turgeon B.G., Tyler B.M., Vincent D., Weissenbach J., Amselem J., Quesneville H., Oliver R.P., WinckerP., Balesdent M.H., Howlett B.J. (2011). Effectors diversification within compartments of the Leptosphaeria maculans genome affected by RIP mutations. Nature communications  2: 202.  https://doi.org/10.1038/ncomms1189

Kale S.D., Gu B., Capelluto, D.F.S., Dou D., Cronin A., Arredondo F.D., Feldman E., Fudal I., Rouxel T., Lawrence C.B., Shan W. and Tyler B.M. (2010). External Lipid PI-3-P Mediates Entry of Eukaryotic Pathogen Effectors into Plant and Animal Host Cells. Cell 142: 284-295.https://doi.org/10.1016/j.cell.2010.06.008

Fudal I., Ross S., Brun H., Besnard A.L., Ermel M., Kuhn M.L., Balesdent M.H. and Rouxel T. (2009). Repeat-induced point mutation (RIP) as an alternative mechanism of evolution towards virulence in Leptosphaeria maculans. Molecular Plant-Microbe Interactions  22 (8): 932-941. https://doi.org/10.1094/MPMI-22-8-0932

Parlange, F.; Daverdin, G.; Fudal, I.; Kuhn, M.L.; Balesdent, M.H.; Blaise, F.; Grezes-Besset, B.; Rouxel, T. (2009). Leptosphaeria maculans avirulence gene AvrLm4-7 confers a dual recognition specificity by Rlm4 and Rlm7 resistance genes of oilseed rape, and circumvents Rlm4-mediated recognition through a single amino acid change. Molecular Microbiology  71(4):851-63. https://doi.org/10.1111/j.1365-2958.2008.06547.x

Collemare J., Pianfetti M., Houille A.E., Morin V., Camborde L., Gagey M.J., Barbisan C., Fudal I., Lebrun M.H., Böhnert H.U. (2008). Magnaporthe grisea avirulence gene ACE1 belongs to an infection-specific gene cluster involved in secondary metabolism. New Phytologist 179 (1), 196-208. https://doi.org/10.1111/j.1469-8137.2008.02459.x

Fudal I., Ross S., Gout L., Blaise F., Kuhn M.L., Eckert M.R., Cattolico L., Bernard-Samain S., Balesdent M.H. and Rouxel T. (2007). Heterochromatin-like regions as ecological niches for avirulence genes in Leptosphaeria maculans genome: map-based cloning of AvrLm6. Molecular Plant-Microbe Interaction 20: 459-470. https://doi.org/10.1094/MPMI-20-4-0459

Fudal I., Collemare J., Böhnert H.U., Melayah D. and Lebrun M.H. (2007). Expression of Magnaporthe grisea avirulence gene ACE1 is connected to the initiation of appressorium-mediated penetration. Eukaryotic Cell 6:546-554. https://doi.org/10.1128/EC.00330-05

Gout L., Fudal I., Kuhn M.L., Blaise F., Eckert M., Cattolico L., Balesdent M.H. and Rouxel T. (2006). Lost in the middle of nowhere: the AvrLm1 avirulence gene of the dothideomycete Leptosphaeria maculans. Molecular Microbiology  60: 67-80. https://doi.org/10.1111/j.1365-2958.2006.05076.x

Fudal I., Böhnert H.U., Tharreau D., and Lebrun M.H. (2005). Transposition of MINE, a composite retrotransposon, in the avirulence gene ACE1 of the rice blast fungus Magnaporthe grisea. Fungal Genetics and Biology  42: 761-772. https://doi.org/10.1016/j.fgb.2005.05.001

Couch B.C., Fudal I., Lebrun M.H., Tharreau D., Valent B., van Kim P., Kohn L.M. (2005). Origins of host specific populations of the blast pathogen, Magnaporthe oryzae, in crop domestication with subsequent expansion of pandemic clones on rice and weeds of rice. Genetics  170: 613-630. https://doi.org/10.1534/genetics.105.041780

Böhnert H.U., Fudal I., Dioh W., Tharreau D., Notteghem J.L. and Lebrun M.H. (2004). A putative polyketide synthase / peptide synthetase from Magnaporthe grisea signals pathogen attack to resistant rice. The Plant Cell16: 2499-2513. https://doi.org/10.1105/tpc.104.022715