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Séminaire - 13 juillet

Séminaire - 13 juillet
Ross Bicknell (New Zealand, Plant and food Research): "Exploring the use of transposon mobilisation to produce a gene-tagged population for grapevine"

Dr Ross Bicknell leads a team studying the genetics of grape with a focus on developing plant materials that improve sustainable grape production in New Zealand. His talk will focus on two projects in his lab; the use of transposons to develop a gene-tagged population in grape, and the development of rootstocks that are resistant to feeding by mealy bugs – a vector of grapevine leaf roll viruses.

 
Using Transposons to produce a gene-tagged population for grapevine. Also breeding for virus vector control.
- Utilisation des transposons pour produire une population génétiquement marquée chez la vigne et amélioration du contrôle des vecteurs de virulence
(Ross Bicknell, Chris Winefield, Darrell Lizamore, Tim Millar,Susan Thomson, Philippa Barrell)

The grape genome is estimated to contain approximately 240,000 transposable elements (TEs), representing 50% of the genetic material within the nucleus. Building on our observation that the transcription of several common retrotransposon classes can be stimulated by stress we are conducting a feasibility study on the use of TE mobilisation to create a gene-tagged population in this plant. Stress treatments are applied to embryogenic callus to reduce the influence of chimerism. Plants are then regenerated and raised through to field planted vines to assess the influence of the treatments at both the genotypic and phenotypic levels. Reduced representation fragment libraries are being sequenced and novel software has been developed to quantify and locate any new insertion sites within the genome of each regenerant. The talk will describe the results of our studies to optimise somatic embryogenesis, the impact of different stress treatments on TE transcription, the establishment of the reduced representation sequencing approach and the development and results obtained using our novel bioinformatics pipeline.

Séminaire à 13H30 dans la grande salle de BIOGER.